Inhibitory Effects of Bay41-4109 on Hepatitis B Virus in vitro

Objective To investigate the anti-hepatitis B virus(HBV) effect of Bay41-4109 on the secretion of HBsAg,HBeAg,HBV DNA in 2.2.15 cells.Methods 2.2.15 cells were cultured and treated with different concentrations of Bay41-4109 for 6 days.The cytotoxicity activity of drugs was determined by methyl thiazolyl tetrazolium(MTT) colorimetric assay,the concentrations of HBsAg and HBeAg in the culture supernatant were detected by real-time enzyme-linked immunosorbent assay(ELISA),and the extracellular HBV DNA was measured by real-time polymerase chain reaction(RT-PCR).Results The toxicity of Bay41-4109 on 2.2.15 cells increased from 12.5 to 50 μM with TC50 of 35.43 μM and TC0 of 12.50 μM.Bay41-4109 had dose-dependent inhibition effect on HBeAg secretion with IC50 of 8.22 μM.The secretion of HBsAg was not inhibited 6 days after Bay41-4109 treatment.The extracellular HBV DNA level of 2.2.15 cells was significantly reduced after Bay41-4109 treatment,and this inhibitory effect was not dose-dependent.Conclusion Bay41-4109 can inhibit the secretion of HBeAg and replication of HBV DNA in 2.2.15 cells.