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2003•Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque ShanghaiRequires access

Determination of Adenosine in Selaginella Tarmariscina by RP-HPLC

Shi She-po

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Abstract

In order to study index composition of quality control in Selaginella tarmariscina, reversed-phase high-performance liquid chromatography was established by choosing SHIM-PACK CLC-ODS column, with water-methanol (100 : 2) as mobile phase and the detection wavelength at 260nm, to determine adenosine contents in Selaginella tarmariscina under room temperature, and also to determine adenosine contents in Selaginella tarmariscina from different places. The results showed that the liner range was 1～8μg; the correlation coefficient was 0.9993; average recovery rate was 96.70% and RSD was 0.93%. The conclusion is that this method is sensitive, simple, convenient and accurate.

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What this paper is about

In order to study index composition of quality control in Selaginella tarmariscina, reversed-phase high-performance liquid chromatography was established by choosing SHIM-PACK CLC-ODS column, with water-methanol (100 : 2) as mobile phase and the detection wavelength at 260nm, to determine adenosine contents in Selaginella tarmariscina under room temperature, and also to determine adenosine contents in Selaginella tarmariscina from different places. The results showed that the liner range was 1～8μg; the correlation coefficient was 0.9993; average recovery rate was 96.70% and RSD was 0.93%. The conclusion is that this method is sensitive, simple, convenient and accurate.

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Available abstract

In order to study index composition of quality control in Selaginella tarmariscina, reversed-phase high-performance liquid chromatography was established by choosing SHIM-PACK CLC-ODS column, with water-methanol (100 : 2) as mobile phase and the detection wavelength at 260nm, to determine adenosine contents in Selaginella tarmariscina under room temperature, and also to determine adenosine contents in Selaginella tarmariscina from different places. The results showed that the liner range was 1～8μg; the correlation coefficient was 0.9993; average recovery rate was 96.70% and RSD was 0.93%. The conclusion is that this method is sensitive, simple, convenient and accurate.

Key concepts: Selaginella, Chromatography, High-performance liquid chromatography, Adenosine, Chemistry, Correlation coefficient, Recovery rate, Mathematics

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