Investigating the molecule mechanism of combination of lactobacilli strain with clostridium butyricum strain on experimental ulcerative colitis

Aim To investigate the molecule mechanism of combination of lactobacilli strain with clostridium butyricum stra on experimental ulcerative colitis.Methods To observed the effects of lactobacilli strain with clostridium butyricum strain,the mice ulcerative colitis was induced by Dextran sulfate Sodium(DSS) and tissue biochemical indexes and the expression of Caspase-1 and PCNA in the colonic mucosa were detected.Results Lactobacilli strain and clostridium butyricum strain significantly abbreviated the damage of colonic tissue and suppressed the expression of Caspase-1 and stimulated the expression of PCNA.moreover,there were the lightest histological damage and the lowest expression of Caspase-1and the highest expression of PCNA in lactobacilli strain combined with clostridium butyricum strain.Conclusions Both lactobacilli strain and clostridium butyricum strain show therapeutic effect on DSS induced mice ulcerative colitis.The coeffects on ulcerative colitis were observed in combination of lactobacilli strain with clostridium butyricum strain.There are lower expression of Caspase-1and higher expression PCNA in the combination group than that in the two strain group alone,which may be the molecule mechanism of the coeffect on mice UC.