Influence of combined administration of lactobacilli and clostridium butyricum on acute mice ulcerative colitis

Objective To investigate the therapeutic effect of combined administration of lactobacilli and clostridium butyricum on acute mice ulcerative colitis,and explore its therapeutic mechanism. Methods Dextran sulfate sodium(DSS)-induced mice model of acute ulcerative colitis was established. After administration of lactobacilli and clostridium butyricum,the pathological change of tunica mucosa coli was observed and the expression levels of tumor necorisis factor(TNF-α)and tissue factor(TF)were measured.Results Lactobacilli and clostridium butyricum significantly alleviated the damage of tunica mucosa coli and suppressed the expression of TNF-αand TF.By comparison,there were the lightest histological damage and the lowest expression of TNF-αand TF when lactobacilli and clostridium butyricum were administrated combined.Conclusion Both lactobacilli and clostridium butyricum show therapeutic effect on DSS-induced mice ulcerative colitis.The coordinate repression on expression of TNF-αand TF may be the molecular mechanism of the co-effect on mice UC.