The antibiotic-resistance and its gene analysis of extended-spectrum β-lactamases in imipenem-resistant Pseudomonas aeruginosa

Objective: To investigate the antimicrobial susceptibility of Pseudomonas aeruginosa and extended-spectrum β-lactamase( ESBLs) producing condition,gene distribution and epidemiological characteristics in clinic. Methods: The antimicrobial susceptibility was detected with the Vitek-2 compact automatic bacteria identification system,16 antibiotics for 208 Pseudomonas aeruginosa isolated.The imipenem-resistant Pseudomonas aeruginosa were selected. ESBLs genes produced by imipenem resistance were detected by PCR,the ESBLs genes contain TEM,SHV,OXA-10,OXA-2,PER and VEB. Results: In the 208 isolates,53 strains of Pseudomonas aeruginosa were resistant to imipenem,the drug resistance rate was 25. 48%. The isolates had a high rate of drug-resistance against CTE,CZ,AMS and FTN; The PCR results showed that in the 53 imipenem-resistant Pseudomonas aeruginosa,ESBLs gene were produced in 23 strains,16 strains were TEM,4 for OXA-10 and 3 for PER gene; OXA-2,SHV was not detected. Conclusions: TEM of Extended-spectrumβ-lactamase producing in imipenem-resistant Pseudomonas aeruginosa is the main gene in our hospital,most of them are multi-drug-resistance,detecting the antimicrobial susceptibility and using antibiotics rationally is essential in clinic.