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2008•Journal of Jiangsu UniversityRequires access

Analysis on drug resistance pattern and genes of β-lactamase of 34 strains multidrug-resistant Pseudomonas aeruginosa

Jing Cheng

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Abstract

Objective:To investigate drug resistance pattern and β-lactamase genic of multidrug-resistant Pseudomonas aeruginosa,so as to provide reference for reasonable selection of antimicrobials.Methods: Antimicrobial susceptibility was evaluated by disk diffusion test(K-B method).The β-lactamase genes: TEM,SHV,VEB,PER carried on multidrug resistant Pseudomonas aeruginosa were detected by polymerase chain reaction method(PCR). Results: The resistant rates of 34 strains multidrug Pseudomonas aeruginosa to 12 kinds of antimicrobials were from 20.5% to 100%.The positive rate of β-lactamase genes TEM,SHV,VEB,PER expression was 44.1%(15/34),11.8%(4/34),5.9%(2/34),and 0%. Conclusion: TEM type β-lactamase was the main type of Pseudomonas aeruginosa gene in our hospital.

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Objective:To investigate drug resistance pattern and β-lactamase genic of multidrug-resistant Pseudomonas aeruginosa,so as to provide reference for reasonable selection of antimicrobials.Methods: Antimicrobial susceptibility was evaluated by disk diffusion test(K-B method).The β-lactamase genes: TEM,SHV,VEB,PER carried on multidrug resistant Pseudomonas aeruginosa were detected by polymerase chain reaction method(PCR). Results: The resistant rates of 34 strains multidrug Pseudomonas aeruginosa to 12 kinds of antimicrobials were from 20.5% to 100%.The positive rate of β-lactamase genes TEM,SHV,VEB,PER expression was 44.1%(15/34),11.8%(4/34),5.9%(2/34),and 0%. Conclusion: TEM type β-lactamase was the main type of Pseudomonas aeruginosa gene in our hospital.

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Available abstract

Objective:To investigate drug resistance pattern and β-lactamase genic of multidrug-resistant Pseudomonas aeruginosa,so as to provide reference for reasonable selection of antimicrobials.Methods: Antimicrobial susceptibility was evaluated by disk diffusion test(K-B method).The β-lactamase genes: TEM,SHV,VEB,PER carried on multidrug resistant Pseudomonas aeruginosa were detected by polymerase chain reaction method(PCR). Results: The resistant rates of 34 strains multidrug Pseudomonas aeruginosa to 12 kinds of antimicrobials were from 20.5% to 100%.The positive rate of β-lactamase genes TEM,SHV,VEB,PER expression was 44.1%(15/34),11.8%(4/34),5.9%(2/34),and 0%. Conclusion: TEM type β-lactamase was the main type of Pseudomonas aeruginosa gene in our hospital.

Key concepts: Pseudomonas aeruginosa, Multiple drug resistance, Microbiology, Antimicrobial, Biology, Drug resistance, Polymerase chain reaction, Pseudomonas

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