Experimental study on the protein expression of NMDAR1 in hippocampus of elderly epilepsy rats induced by kainic acid

Objective By observing the protein expression of N-methyl-D-aspartate receptor(NMDAR)1 in hippocampus of the elderly epilepsy rats to explore its mechanism.Methods Epilepsy rat models were made by injecting kainic acid into amygdala under stereotactic instrument.60 male Wistar rats were divided randomly into normal control group,false operation group and epilepsy group.NMDAR1 protein expression were obtained at different time and tested by immunohistochemistry and FAC scan flow cell analysis.Results In the normal group and false operation group,NMDAR1 positive cells distributed rarely in hippocampus;NMDAR1 positive cells of epilepsy groups were significantly higher 2 h later,reached peak at 6 h,persisting to 24 h(P≤0.01).Conclusions NMDAR1 protein expression is up-regulated in epilepsy rats brain and persists for 24 h,which could participate in the excitation process of long time course in brain of the epilepsy rats.