2013•Liaoning Zhongyiyao Daxue xuebaoRequires access

Determination of Tetrahydropalmatine of Yuanhu Zhitong Suppository by HPLC

Ziyang Cheng

Abstract

Objective:To develop a RP-HPLC method for the determination of tetrahydropalmatine in Yuanhu Zhitong suppository.Methods:The separation was performed at 30 ℃ on an Agilent Zorbax 80A Extend-C18 column(150 mm×4.6 mm,5 μm)with a mobile phase of methanol-water-triethylamine(55∶45∶0.1).The flow rate was 1.0 mL·min-1,and the detecting wavelength was 280 nm.Results:The linear range of tetrahydropalmatine was from 19.5 to 97.5 μg·mL-1,the average recovery(n=6)of tetrahydropalmatine was 100.6%(RSD=1.07%).Conclusion:The method is simple and accurate with good reproducibility.It can be used to control the quality of Yuanhu Zhitong suppository.

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Available abstract

Key concepts: Tetrahydropalmatine, Suppository, Medicine, Chromatography, Triethylamine, High-performance liquid chromatography, Reproducibility, Content determination