2011•Zhongguo zhongyiyao xinxi zazhiRequires access
HPLC Determination of Tetrahydropalmatine in Yuanhu Zhitong Drop Pills
Zhu Yu
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Abstract
Objective To establish a method for determination of Tetrahydropalmatine in Yuanhu Zhitong Drop Pills.Methods Tetrahydropalmatine in Yuanhu Zhitong Drop Pills was determined by HPLC.CAPCELL PAK C18(4.6 mm×250 mm,5 μm) column was used.The modbile phase was metanol∶0.02% potassium dihydrogen phosphate-0.96% potassium hydrogen phosphate(adjusted pH to 6.5 with phosphoric acid)=55∶45,with the flow rate of 1.0 mL/min.The column temperature was 40 ℃ and UV detection wavelength was at 280 nm.Results Tetrahydropalmatine was linear at the range of 0.110-0.825 μg.The average recovery was 97.39% and RSD was 2.02%(n=6).Conclusion The established method is accurate,reliable and specific.The results are stable with good reproducibility.The quality of Yuanhu Zhitong Drop Pills can be controlled by the method.