Tamoxifen combined with cisplatin suppress the proliferation of human ovarian cancer cell line HO-8910 in vitro

Purpose:To investigate the effect of tamoxifen combined with cisplatin on the growth of human ovarian cancer cell,and to insepect the efficacy that TAM combined with DDP suppressed the growth and proliferation of the cell HO-8910 so as to provide theoretical evidence of the combination with endocrine therapy and chemotherapy.Methods:Human ovarian cancer cell line HO-8910 was studied.Different doses of TAM and DDP were combined in human ovarian cancer cell line HO-8910.The growth inhibition ratio of the cell was detected with MTT,the expression of Prolifering cell nuclear antigen(PCNA) in HO-8910 was detected with immunohistochemical stining SP method,the cell cycle changes was analyzed by PI coloration of flow cytometry and cell apoptosis index was analyzed by AV-PI coloration of flow cytometry.Results:Compared with positive control group,the experimental groups of high-dose TAM combined with different dose of DDP and the experimental group of intermedial dose TAM combined with high-dose DDP(3.3μmol/L) had significant difference in the growth inhibition ratio of the cell(P0.05);TAM(≥1.0μmol/L) combined with different dose of DDP had significant difference in the expression of PCNA(P0.05);the most prominent effect of TAM on cell cycle kinetics was aslowdown in G_1-phase transit and DDP induced S-phase arrest;following the increase of doses of TAM and DPP apoptotic index increased by degrees.Compared with the groups of drug combination,it was the experimental group of high-dose TAM combined with low-dose DDP(1.7μmol/L) and the group of intermedial dose TAM combined with high-dose DDP(3.3μmol/L) that had significant difference in the growth inhibition ratio of the cell and the expression of PCNA.Conclusion:TAM combined with DDP suppressed the growth and proliferation of the cell HO-8910 efficiently.