Effect of tea ployphenols combined with cisplatin on SKOV3 ovarian cancer cell line and the mechanism

Objective To study the effect and mechanism of tea ployphenols(TP),cisplatin(DDP)alone and in combination upon the proliferation and cell apoptosis of human ovarian cancer cells SKOV3 in vitro.Methods SKOV3 cells were treated with 6.25 μg/ml TP,2.5 μg/ml DDP,and 6.25 μg/ml TP plus 2.5 μg/ml DDP.Cell proliferation was determined by MTT assay,the morphology of apoptotic cells was observed by DAPI staining,and Annexin V-FITC/PI double staining flow cytometry was used to analyze apoptosis.The expression of Akt and Akt phosphorylation were detected by Western Blot.Results The inhibiting rates of cells treated with the single TP,DDP,and TP plus DDP for 48 h were(11.47±2.07)%,(32.26±4.85)% and(52.62±3.23)%,respectively;the antiproliferation effect of TP combined with DDP group was superior to single DDP group(P=0.000).The results of DAPI staining showed that apoptotic changes including karyorrhexis and karyopyknosis appeared after treatment of single DDP and in combination with TP,and the apoptotic changes in TP combined with DDP group was more obvious than those in single DDP group.Low doses of TP had no significant apoptosis-promoting effect,but the combined treatment group apoptosis rate was significantly higher than the DDP single group.Western blot analysis showed that single groups and TP combined with DDP both unchanged the expression of total Akt protein in human ovarian cancer SKOV3 cells,reduced the expression of Akt phosphorylation,and the effect was more obvious in TP combined with DDP group.Conclusions TP combined with DDP can synergistically inhibit the proliferation of SKOV3 ovarian cancer cell line,induce cell apoptosis,which may be associated with the reduction of AKT phosphorylation.