The effect of tamoxifen combined with medroxyprogesterone on the growth of cisplatin-resistant human epithelial ovarian cancer cell oine SKOV -3/DDP

Objective: To investigate the effect of tamoxifen combined with medroxyprogesterone on the growth of cisplatin -resistant human epithelial ovarian cancer cell line SKOV - 3/DDP. Methods: SKOV - 3/DDP cells were treated with different concentrations (1× 10-10-1×10-4mol/L) of tamoxifen combined with medroxyprogesterone. . The inhibition of SKOV-3/DDP cell growth was measured by MTT. The morphological change of cell was observed by electron microscopy. Cell cycle and apoptotic percentage were accounted by flow cytometry. Results: Low concentration (1×10-10 -1×10-7mol/L) of tamoxifen combined with medroxyprogesterone had no effect on SKOV-3/DDP cell growth. SKOV -3/DDP cell growth was inhibited by drugs at high concentration (1×10-6-1×10-4mol/L) in a dose-dependent manner (P0. 01) . With the high concentration (1×10-6-1×10-4mol/L) of drugs, GO/G1 arrest of SKOV-3/DDP cell was enhanced and the apoptotic peak and percentage was increased. Conclusion: Tamoxifen and medroxyprogesterone with the inhibitory effect on the growth of cisplatin-resistant ovarian cancer cells and the minor ill effect could be used to ovarian cancer patients as an adjuvant therapy.