Study on Optimizing Conditions for Chemical Hydrolysis and Concentration of EPA and DHA in Fish Oil
Yu Hui
Abstract
Yu Hui
Abstract
The optimizing conditions of chemical hydrolysis in fish oil and separation ofω-3 polyunsaturated fatty acids (EPA/DHA)from hydrolysis fish oil were studied,respectively. The results showed:the optimum conditions:saponification time 60 min,50 ℃,ethanol/water 6:1 (v/v),the degree of hydrolysis was 95.5% and the recovery of EPA and DHA were 91.2% and 88.9%. C18 column was used to isolate EPA/DHA in hydrolysis fish oil,the purity of final product of EPA/DHA was proved 92.9% and 93.2% respectively by MS and HPLC analysis.
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The optimizing conditions of chemical hydrolysis in fish oil and separation ofω-3 polyunsaturated fatty acids (EPA/DHA)from hydrolysis fish oil were studied,respectively. The results showed:the optimum conditions:saponification time 60 min,50 ℃,ethanol/water 6:1 (v/v),the degree of hydrolysis was 95.5% and the recovery of EPA and DHA were 91.2% and 88.9%. C18 column was used to isolate EPA/DHA in hydrolysis fish oil,the purity of final product of EPA/DHA was proved 92.9% and 93.2% respectively by MS and HPLC analysis.
Key concepts: Fish oil, Hydrolysis, Saponification, Chemistry, Polyunsaturated fatty acid, Chromatography, Docosahexaenoic acid, Fish <Actinopterygii>