Expression of CD146 in ectopic and eutopic endometrium of patients with adenomyosis

Objective:To observe the relationship between expression levels of CD146 in ectopic and eutopic endometrium of patients with adenomyosis and angiogenesis of microvessels,explore the changes of angiogenesis of microvessels in patients with adenomyosis.Methods:66 patients undergoing hysterectomy were divided into adenomyosis group (40 cases) and control group (26 cases),the expression levels of CD146,amount of microvessels and the values of microvessel density (MVD) in ectopic and eutopic endometrium of patients with adenomyosis and normal endometrium were detected by immunohistochemical staining.Results:In eutopic endometrium of adenomyosis group,the value of MVD in secretory phase was (33.7±4.9),which was significantly higher than that in proliferative phase (27.2±5.0) (P0.01),there was no significant difference in value of MVD between secretory phase (20.5±3.8) and proliferative phase (17.9±3.6) (P0.05).The value of MVD in eutopic endometrium of adenomyosis group was significantly higher than that in control group in proliferative phase,secretory phase and throughout the menstrual cycle (P0.01).In adenomyosis group,there was significant difference in expression level of CD146 between eutopic endometrium (4.1±1.4) and ectopic endometrium (4.9±1.4) (P0.05),compared to that in proliferative phase of eutopic endometrium (3.6±1.7),there was significant difference,compared to that in secretory phase (4.7±0.6),the level increased,but there was no significant difference (P0.05);compared to the whole menstrual cycle (2.4±0.8),proliferative phase (2.4±0.7) and secretory phase (2.3±0.9),the expression levels of CD146 increased.There was significant difference in positive expression rate of CD146 between eutopic endometrium (85%,34/40),ectopic endometrium (90%,36/40) in adenomyosis group and control group (62%,16/26) (P0.01).There was a positive correlation between CD146 and MVD (γ=0.567,P0.01).Conclusion:The expression of CD146 in eutopic and ectopic endometrium of adenomyosis increased significantly,which indicates that CD146 plays an important role in angiogenesis of microvessels in patients with endometriosis.