Expression of ki67 and CA_(125)in Adenomyosis

Objective: ① To investigate the proliferative activity and expression of cell proliferation-associated nuclear antigen ki67 in the pathogenesis of adenomyosis uturus. ② And to study the source and significance of increased CA 125 in adenomyosis. Methods: 27 patients with histologically proven adenomyosis and 32 patients with leiomyoma of the uterus were included. Immunohistochemical staining was used to detect of expression CA 125 and ki67 in eutopic and ectopic endometrium of adenomyosis and in eutopic endometrium from women with leiomyoma. Serum CA 125 levels were determined by chemiluminescent enzyme immunometric assay. Results: The ki67 staining intensity of glandular cells in the endometrium of the two groups in the proliferative phase were stronger than secretory phase,(P0.05), but no difference was shown between two groups. Ectopic endometrium showed no cyclic change, and stronger staining intensity was found in the glandular cells than that of eutopic endometrium in luteal phase. The median serum CA 125 level in adenomyosis group was significantly higher than that in leiomyoma group(P0.001), but no significant difference was shown between the proliferative and secretory phase. The CA 125 staining intensity of glandular cells in ectopic endometrium of adenomyosis was similar to that of eutopic endometrium , no cyclic change was found. Conclusions: ① The ki67 staining intensity of ectopic endometrium was stronger than that of eutopic endometrium, the proliferative activity of the endometrium may play significant effects on the development of the adenomyosis after the invasion into the myometrium. ② The median serum CA 125 level inpatients with adenomyosis was significantly higher than that of leiomyoma.