Effect of liposome-mediated rhIGF-1 gene on proliferation and differentiation of osteoblast

Objective To study the effects of the insulin-like growth factor-1(IGF-1) gene on proliferation and differentiation of rat osteoblast in vitro.Methods Osteoblast were obtained from within 24 hours newborn rats cranium through trypsin and collagenase digestion.Identified the of the third subculture cells osteoblast quality,the mineralization nodes were stained by Alizarin Red S (ARS),Gomori method show the expression of alkaline phoaphatase(ALP)in osteoblast.pcDNA3.1-rhIGF-1 plasmid were transfected into osteoblast by lipofectamine.Morphologic changes of osteoblast were observed,the proliferation were detected by MTT method,the activity of ossification were studied by alkaline phoaphatase(ALP) and osteocalcin(OCN).Results Established the stable model of rat osteoblast.Comparing with control and void plasmid group,pcDNA3.1-rhIGF-1 plasmid group obviously promoted the proliferation of osteoblast and increased ALP and OCN secretion,the differences were significant(P(0.01)).Conclusion Exogenous rhIGF-1 gene could stimulate proliferation and differentiation of osteoblast and promote bone formation.