Effects of mesangial cell on proliferation and function of osteoblast in vitro

Objective:To explore the effects and mechanism of mesangial cell(MC) on proliferation and function of osteoblast in vitro from growth factors.Methods:Osteoblast cultured in vitro were divided into normal blood serum group and MC group and cultured supernatant fluid by 10% serum culture medium and 20% MC culture medium.MTT was used to checked the proliferation of osteoblast at the end of 24,48,72,120 h;Expression of BGP and OPN were tested 72 h and 144 h after culture;Expression of IGF-α and TGF-β were tested 144 h after culture.Results:The proliferation of osteoblast in MC group was obviously higher than normal blood serum group in different times(P0.05);The level of BGP and OPN of osteoblast was obviously higher than normal blood serum group(11.3%,16.4%,55.0% and 39.6%);and the level of IGF-α and TGF-β of osteoblast was obviously higher than normal blood serum group(10.1% and 47.7%).Conclusion:MC can directly act on osteoblast,promote the proliferation and function of osteoblast by promoting secretion of TGF-β.