The effect of colleganase A(MMP-2) in judging the progress of transitional cell carcinoma of bladder

Objective To study on the expression of Colleganase A (MMP-2) in different grade and stage transitional cell carcinoma of bladder(TCCB), and to find the possibility and method to judge the progress of TCCB through MMP-2. Method Immunohistochemistry and zymography were used to study the content of MMP-2 in 47 surgically resected specimens from patients with TCCB, and 5 normal bladder tissue were obtained as control,then statistical methods were used to analyse the data. Result (1) MMP-2 protein appeared in TCCB parenchyma,but it was weak or negative in normal control group. (2)The content of positive expression of MMP-2 were significantly increased with stage and grade ( P 0.01,respectively). Conclusion The result showed that the expression of MMP-2 are significantly correlated with the malignant of TCCB. MMP-2 protein could be used as an important index to judge the progress of TCCB in clinical work.