Protective effects and mechanisms of ulilnastatin against renal injury in rats with diabetic nephropathy

Objective To investigate protective effects of ulilnastatin(UTI) on kidneys of diabetic rats and its mechanisms.Methods 10 rats were randomly selected from 30 Wistar rats as the control group(group A).The remaining 20 rats injected with streptozotocin(STZ) were randomly divided into the diabetic group(group B) and the UTI group(group C),10 rats in each group.Rats in group C were intraperitoneally administered UTI at a dose of 40 000 IU/kg,and rats in groups A and B were intraperitoneally administered the same volume of normal saline.At the 8th and 12th weeks,fasting blood glucose(FPG),urine creatinine and serum creatinine(SCr) were detected,24h urine was collected,the albumin was detected,and creatinine clear rate(Ccr) was calculated.Nephridial tissue homogenate was made and malondialdehyde(MDA),superoxide dismutase(SOD),connective tissue growth factor(CTGF),matrix metallopeptidase-9(MMP-9) and plasminogen activator inhibitor-1(PAI-1) in serum and nephridial tissue homogenate were detected.Results ① FBG in groups B and C was obviously more increased than in group A(P0.05),while there was no significant difference between group C and group B(P0.05).② 24h urinary albumin and Scr were obviously more increased in groups B and C than in group A(P0.05),and they were obviously more decreased in group C than in group B(P 0.05).③ Ccr in groups B and C was obviously more decreased than in group A(P0.05),and that in group C was obviously more increased than in group B(P0.05).④ In contrast to group A,MDA,CTGF and PAI-1 in groups B and C were obviously more increased(P0.05),and those in group C were obviously more decreased than group B(P0.05).⑤ SOD and MMP-9 in groups B and C were obviously more decreased than in group A(P0.05),those in group C were obviously more increased than in group B(P0.05).Conclusion UTI exerts protective effects on renal function of diabetic rats,and the mechanism of this action is related to decreases of urinary albumin,MDA,CTGF and PAI-1,and increases of SOD and MMP-9.