Analysis of Antibitotic Resistance of ESBLs-product Escherichia Coli and Klebsiella Pneumoniae

OBJECTIVE To investigate the trend of antibiotic resistance of extend-spectrum β-lactamases(ESBLs) producing Escherichia coli and Klebsiella pneumoniae in our hospital,and to provide a guidance for clinical rational use of antibiotics.METHODS Escherichia coli and Klebsiella pneumoniae strains were collected,which were isolated from patients in clinical in 2008-2009.Bacterial susceptibility testing were detected by MIC methods and ESBLs-producing strains were detected by double-disc diffusion confirming test.RESULTS In 2008,312 of 764 strains of Escherichia coli were considered ESBLs,the rate of ESBLs were 40.8%;133 of 356 strains of Klebsiella pneumoniae were considered ESBLs,the rate of ESBLs were 37.4%.In 2009,246 of 499 strains of Escherichia coli were considered ESBLs,the rate of ESBLs were 49.3%;107 of 298 strains of Klebsiella pneumoniae were considered ESBLs,the rate of ESBLs were 35.9%.The resistance rate of ESBLs-producing strains were significantly higher than that of ESBLs non-producing strains(P0.05).The resistance rate of ESBLs-producing strains to aztreonam and cephalosporin were higher in 2009 than in 2008(P0.05).The resistance rate of ESBLs-producing strains to quinolones were lower in 2009 than in 2008(P0.05).The resistance rate of ESBLs-producing strains of Klebsiella pneumoniae to imipenem was increased by 15.9% in 2009(P0.05).CONCLUSION ESBLs producing Escherichia coli and Klebsiella pneumoniae have the multidrug-resistant phenomenon,surveillance should be intensified,so as to avoid the increasing,diffusive and popular of ESBLs-producing bacteria.