Optimization of PB011 lipase-catalyzed synthesis of biodiesel from soybean oil by response surface method

PB011 lipase was used to catalyze soybean oil to produce biodiesel.The influences of enzyme dosage,reaction temperature,organic solvent dosage,substrate molar ratio,adding times and water content on transesterification reaction were researched,and the reaction conditions were optimized by response surface method(RSM).The results showed that the optimum reaction conditions were as follows:enzyme dosage 11.5%,reaction temperature 37.5℃,adding times of methanol 4,solvent dosage 19 mL,water content 2.5%,substrate molar ratio 1∶1.Under the best conditions,the conversion rate of FAME can reach 96.89%.