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2008•Zhongguo shiyan fangjixue zazhiRequires access

Determination of Baicalin and Wogonin in Tongjing Patch by HPLC

Xiaoyan Zhang

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Abstract

Objective:To develop an HPLC method to determine the contents of both baicalin and wogonin in Tongjing Patch.MethodA Zorbax SB-C18 column(4.6×250 mm,5 μm)was used.The mobile phase was composed of methanol-water-acetic acid(58∶42∶0.05) and the flow rate was 1.0 ml·min-1.UV detection wave length was set at 278 nm.Results:The linear range of baicalin was(0.44～2.20)μg(r= 0.999 7)and the average recovery was 98.64% and the RSD was 1.17%.The linear range of wogonin was(0.012 8 ～ 0.204 8)μg(r= 0.999 9)and the average recovery was 98.96% and the RSD was 2.08%.In three batches of the patches,the contents of baicalin were 7.33%,6.74% and 7.29% and that of wogonin were 0.71%,0.65% and 0.63%.Conclusion:This method is simple and accurate and can be used for the determination of the contents of both baicalin and wogonin in Tongjing Patch.

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Objective:To develop an HPLC method to determine the contents of both baicalin and wogonin in Tongjing Patch.MethodA Zorbax SB-C18 column(4.6×250 mm,5 μm)was used.The mobile phase was composed of methanol-water-acetic acid(58∶42∶0.05) and the flow rate was 1.0 ml·min-1.UV detection wave length was set at 278 nm.Results:The linear range of baicalin was(0.44～2.20)μg(r= 0.999 7)and the average recovery was 98.64% and the RSD was 1.17%.The linear range of wogonin was(0.012 8 ～ 0.204 8)μg(r= 0.999 9)and the average recovery was 98.96% and the RSD was 2.08%.In three batches of the patches,the contents of baicalin were 7.33%,6.74% and 7.29% and that of wogonin were 0.71%,0.65% and 0.63%.Conclusion:This method is simple and accurate and can be used for the determination of the contents of both baicalin and wogonin in Tongjing Patch.

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Available abstract

Objective:To develop an HPLC method to determine the contents of both baicalin and wogonin in Tongjing Patch.MethodA Zorbax SB-C18 column(4.6×250 mm,5 μm)was used.The mobile phase was composed of methanol-water-acetic acid(58∶42∶0.05) and the flow rate was 1.0 ml·min-1.UV detection wave length was set at 278 nm.Results:The linear range of baicalin was(0.44～2.20)μg(r= 0.999 7)and the average recovery was 98.64% and the RSD was 1.17%.The linear range of wogonin was(0.012 8 ～ 0.204 8)μg(r= 0.999 9)and the average recovery was 98.96% and the RSD was 2.08%.In three batches of the patches,the contents of baicalin were 7.33%,6.74% and 7.29% and that of wogonin were 0.71%,0.65% and 0.63%.Conclusion:This method is simple and accurate and can be used for the determination of the contents of both baicalin and wogonin in Tongjing Patch.

Key concepts: Baicalin, Wogonin, Chromatography, High-performance liquid chromatography, Chemistry, Linear range, Acetic acid, Linear relationship

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