THE INHIBITORY ACTION OF CERIOPS TAGAL EXTRACT Tagalsin ON EHRLICH ASCITES TUMOR IN MICE

Objective To explore the antitumor action of Tagalsin(extracted from Ceriops tagal) and its effect on the expression of bcl-2/bax. Methods Models of Ehrlich ascites tumor were created in rats,which were randomly divided into five groups as follows: high-dose Tagalsin(Tagalsin 16.50 mg·kg-1·d-1),moderate-dose Tagalsin(3.30 mg·kg-1·d-1),low-dose(Tagalsin 0.66 mg·kg-1·d-1),blank-control and positive control.In Tagalsin groups,a corresponding dose of Talgasin was offered;in the blank group,a same volume of edible oil was given;in positive control group,carmofur tablets 100 mg/(kg·d),which were all administrated intragastrically for 10 days.The oncostatic rate,the spleen and thymus indexes were calculated,and the expressions of bcl-2/bax detected by immunohistochemistry. Results Compared with the blank control group,the tumor grew more slowly in the positive control and different-dose Tagalsin groups(F=1 588.82,t=36.38-75.19,P0.05),and the oncostatic rate of the high-,middle-,low-dose and positive control group was 37.25%,34.10%,29.51%,and 61.03%,respectively.Compared with the blank control group,the indexes of spleen and thymus in the positive control and Tagalsin groups were significantly lower;compared with the positive control,the index of thymus in Tagalsin groups was lower(F=95 874.12,t=2.54-474.59,P0.05).The differences of the ratio of bcl-2/bax among the blank control,the positive control and Tagalsin groups were statistically significant(F=342.89,t=23.30-47.19,P0.05). Conclusion Tagalsin inhibits the growth of tumor by decreasing bcl-2 and increasing bax expression,which has a certain regulation action on humoral immunity in mice.