ANTI-TUMOR EFFECT OF Dolabrane DITERPENES IN THE MANGROVE PLANT CERIOPS TAGAL

Objective To study the anti-tumor effect of Dolabrane diterpenes(Tagalsins A) of the mangrove plant ceriops tagal and its mechanism. Methods Models of solid tumor of hepatoma-22(H-22) and ascitic tumor of H-22 were created.All the models were randomized to five groups: high-dose,medium-dose,and low-dose Tagalsins A groups(5,10 and 20 mg·kg-1,respectively),saline group and CTX group(10 mg·kg-1),with 10 mice in each group.The Tagalsins A was given through intragastric administration,the CTX through intraperitoneal injection for 7 days,the same volume of saline was given to the control group.The mice were killed 24 hours after the last medication,the tumor inhibition rate(TIR),life-prolong rate,organ index,and apoptosis rate were calculated. Results Compared with NS group,the growth of H-22 solid tumors in the mice of different-dose tagalsins A groups was significantly inhibited(F=8.90;q=3.31-6.52;P0.01).The TIR in low-,medium-,and low-dose groups were 24.49%,35.37%,and 48.30%,respectively,in which,the differences of TIR among medium-,high-dose,and CTX group were not significant(q=2.85,1.01;P0.05).Tagalsins A significantly prolonged the survival of H-22 ascites tumor-bearing mice(F=17.06;q=5.45-11.26;P0.01),reduced the thymus index(F=21.82,P0.01),while elevated liver index(F=121.84,P0.01).The rates of apoptosis of tumor cells in Tagalsins A groups were higher than that in the NS group(F=127 7.68,q=51.11-82.02,P0.01). Conclusion Tagalsins A manifests an important effect on inhibition of the growth of transplanted H-22 in mice,the mechanism might be related to apoptosis of inducer cells.