[Effect of acrylonitrile on the expression of Bcl-2 and Bax in spermatogenic cell of mice].

OBJECTIVE: To explore the effect of acrylonitrile exposure on the expression of Bcl-2 and Bax in mice spermatogenic cells. METHODS: Based on body weight, 250 SPF Kunming adult male mice were randomly divided into five groups: negative control group (normal saline 0.01ml/g), three AN exposure groups (intraperitoneal injection of 1.25, 2.50 or 5.00 mg/kg of AN daily for 5 days, respectively) and positive control group (intraperitoneal injection of cyclophosphamide 40mg/kg). Mice were killed in the 7th, 14th, 21st, 28th and 35th day after the first exposure by cervical dislocation. Immunohistochemical method (SABC) was used to detect the expression of Bcl-2 and Bax protein in spermatogenic cells. RESULTS: The average optical density values of Bcl-2 at five time points of the AN 2.50 mg/kg group and the 21th day point of the AN 1.25mg/kg group were significantly lower than the negative control group (P < 0.05). Except the 21st day point of the AN 1.25 mg/kg group, the mean optical density values of Bax in all time points of AN exposure groups were significantly higher than the negative control group (P < 0.05). The decreased expression of Bcl-2 protein was most distinct in AN 2.50mg/kg group and the positive control group at all time points. The expression of Bax protein was significantly increased in all groups at the 14th day point. CONCLUSION: The expression of Bcl-2 protein could be weakened in spermatogenic cells induced by AN, especially in the AN 2.50 mg/kg group; while the expression of Bax was enhanced, and the amplitude of change in the 14th day point was more obvious.