Detection of Mycoplasma in 296 Patients with Non-gonococcal Urogenital Tract in Fection and Test of Susceptibility of Ureaplasma Urealytica to Antimicrobial Agents

Objective:To explore the role of Ureaplasma urealytica (Uu) and Mycoplasma hominis (Mh) in non gonococcal urogenital tract infection and examine their in vitro drug susceptibility. Methods:Detection of mycoplasma and susceptibility test of ureaplasma urealytica to 10 antimicrobial agents were performed by using microdilution method. Results:Among the 296 suspected cases, 101(34.1%) were positive with mycoplasma , 75 (28.8%) with Uu, 6(2.0%) with Mh, and 20 (6.8%) with Mh plus Uu. The positive rate with mycoplasma infection was significantly higher among the female subjects (42.9%) than that among the male (28.8%). The drug susceptibility test showed that, among the 10 antimicrobial agents tested, resistance of Uu was most commonly found to spiramycin (100.0%), and least found to josamycine (7.9%). Frequency of resistance of Uu, among the tested cases, to the other antimicrobial agents, was as followes: to ofloxacin (37.3%), to tetracycline (37.3%), minocycline (33.3%), doxycycline (32.0%), erythromycine (30.7%), levofloxacin (18.7%), azithromycin (13.3%), and to roxithromycin (10.7%). The cross resistance to fluoro quinolones , tetracycline and macrolides was found. The highest rate of cross resistance was between fluoro quinolores and tetracycline (12.0%). Conclusion:Monitoring the variance of resistance of mycoplasma is of important significance for guiding the use of drugs.