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Establishment of TRFIA for anti-thyroglobulin antibody and its clinical application

LI Yueson

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Abstract

Objective To establish a time-resolved fluoroimmunoassay(TRFIA)for anti-thyroglobulin antibody(TgAb).Methods The thyroglobulin antigen was coated on the 96 well plates.A goat anti-mouse IgG-europium(Eu3+) was used to the detection.Serum TgAb level was detected by TRFIA.Results The sensitivity was 1.0 IU/mL.The within-run coefficient of variation(CV)was 3.3%-4.1%.The between-run CV was 3.7%-4.7%.The average recovery was 97.9%.The kit was stable.The correlation coefficient with TgAb by electrochemiluminescence immunoassay(ECLIA) was 0.881 3.The TgAb levels between males and females had no statistical significance.The normal range of TgAb was ≤ 110 IU/mL.Conclusions The established TRFIA for the detection of TgAb in serum is a sensitive and reliable assay,which would be useful for the clinical diagnosis of thyroid diseases.

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What this paper is about

Objective To establish a time-resolved fluoroimmunoassay(TRFIA)for anti-thyroglobulin antibody(TgAb).Methods The thyroglobulin antigen was coated on the 96 well plates.A goat anti-mouse IgG-europium(Eu3+) was used to the detection.Serum TgAb level was detected by TRFIA.Results The sensitivity was 1.0 IU/mL.The within-run coefficient of variation(CV)was 3.3%-4.1%.The between-run CV was 3.7%-4.7%.The average recovery was 97.9%.The kit was stable.The correlation coefficient with TgAb by electrochemiluminescence immunoassay(ECLIA) was 0.881 3.The TgAb levels between males and females had no statistical significance.The normal range of TgAb was ≤ 110 IU/mL.Conclusions The established TRFIA for the detection of TgAb in serum is a sensitive and reliable assay,which would be useful for the clinical diagnosis of thyroid diseases.

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Available abstract

Objective To establish a time-resolved fluoroimmunoassay(TRFIA)for anti-thyroglobulin antibody(TgAb).Methods The thyroglobulin antigen was coated on the 96 well plates.A goat anti-mouse IgG-europium(Eu3+) was used to the detection.Serum TgAb level was detected by TRFIA.Results The sensitivity was 1.0 IU/mL.The within-run coefficient of variation(CV)was 3.3%-4.1%.The between-run CV was 3.7%-4.7%.The average recovery was 97.9%.The kit was stable.The correlation coefficient with TgAb by electrochemiluminescence immunoassay(ECLIA) was 0.881 3.The TgAb levels between males and females had no statistical significance.The normal range of TgAb was ≤ 110 IU/mL.Conclusions The established TRFIA for the detection of TgAb in serum is a sensitive and reliable assay,which would be useful for the clinical diagnosis of thyroid diseases.

Key concepts: Thyroglobulin, Medicine, Antibody, Coefficient of variation, Subclinical infection, Immunoassay, Thyroid, Internal medicine

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