Effects of ulinastatin on intestinal mucosal barrier after intestinal ischemia-reperfusion in rats

Objective: To explore the protective effects of a human proteinkinase inhibitor-Ulinastatin(UTI) on intestinal mucosal barrier functions in rats after intestinal ischemia-reperfusion. Methods: The superior mesenteric artery(SMA) of male Wistar rats was occluded for 1h and subsequent reperfusion was for 1 and 2 h.The rats were randomly divided into group A(control) and B(with UTI treatment).UTI(5×10~(4)u/kg) were administered to group B after ischemia from jugular vein by infusion pump,while an equal amount of normal saline were administered to group A.Blood samples were taken for endotoxin、plasma D-lactate、MDA and SOD determination at various intervals.After injection of acridine orange from the alimentary tract,mesenteric lymph node and tissue of liver were taken and homogenated for bacterial count under fluorescent microscope.Intestinal pathology was scored by Chiu′s score system under light microscope. Results: Serum levels of endotoxin、plasma D-lactate、MDA and SOD were higher in group A than that in group B,so was the bacterial count in the mesenteric lymph node and tissue of liver.Intestinal mucosa injury was more serious in group A than in B. Conclusion: UTI can significantly lessen the release of oxyradicals and endotoxin translocation,strengthen the clearance of oxyradicals during intestinal ischemia-reperfusion,and then reduce the intestinal mucosal injury and intestinal mucosal permeability.