The protective effect of ulinastatin on the intestinal mucosal barrier in rats after intestinal ischemia-reperfusion

Objective To investigate the protective effect of ulinastatin on the intestinal mucosal barrier in rats with ischemia-reperfusion.Methods Totally 24 SD rats were randomly divided into 3 groups after clamping superiormesenteric artery for 1 h and reperfusion for 1 h: blank control group,control group and treatment group.Rats in the treatment group were injected with ulinastatin(50 000 U/kg) through vena dorsalis penis after ischemia-reperfusion model was induced.The control group underwent laparotomy with only the manipulation of the intestine and the same dose of saline was used through the same way.Specimens were obtained after ischemia-reperfusion model was induced.Dynamic turbidimetry was used to evaluate the effect of ulinastatin on the intestinal endotoxin translocation in rats.Apoptosis of mucosal cells was detected by TUNEL method,and the expressions of Bax and Bcl-2 mRNA were determined by semi-quantitative RT-PCR.Results The serum endotoxin level and apoptotic index of mucosal cells were evidently lower in blank control group than in control group(P0.01).The expression of Bax mRNA in intestinal mucosal cells was lower in treatment group than in control group(P0.01),but the expression of Bcl-2 mRNA was lower in treatment group(P0.01).Conclusion Ulinastatin can prevent the translocation of bacteria and endotoxin in ischemia-reperfusion injury by inhibiting the apoptosis of intestinal mucosa cells and maintaining the intestinal barrier function.