Protective effects of glucagon-like peptide 2 on intestinal ischemia-reperfusion injury of young rats

Objective To explore the protective effects of glucagon-like peptide 2(GLP-2) on intestinal ischemia-reperfusion(I-R)injury of young rats.Methods Using the rat model of intestinal ischemia-reperfusion,24 young male Wista rats were randomly assigned to three groups of sham operation(group C),I-R(group B) and I-R+GLP-2(group A) with 8 rats each.Intestinal pathology was examined under light microscope.Bacterial colony of mesenteric lymph node(MLN),intestinal mucosal malondialdehyde(MDA),serum D-lactate,endotoxin,and tumor necrosis factoe(TNF)-α were studied.Results The intestinal injury was significant in group B,and Chiu′s scores,bacterial colony counts of MLN,intestinal mucosal MDA,serum D-lactate,endotoxin,and TNF-α were also significantly higher in group B than those in group C(P0.01),which were significantly lower in group A than those in group B(P0.05).Conclusion GLP-2 attenuates intestinal I-R injury,protects intestinal mucosal barrier,reduces bacterial/endotoxin translocation,inhibits the release of oxygen free radicles and inflammatory cytokines.