Relationship between apolipoprotein H gene polymorphism with coronary heart disease

Objective To investigate relationship between the apolipoprotein H(ApoH) exon3 and exon 8 polymorphism with coronary heart disease(CHD).Methods Polymerase chain reaction and technique of restriction fragment length polymorphism were used to determine the genotypes of ApoH exon 3 and exon8 in 110 patients with CHD and 100 health control group.Results The genotype GG of ApoH in the CHD group was 81.8%,GA+AA genotype was 11.8% only,the frequency of G allele was 88%,while 12%in the A allele,there was no significant difference with the control groups,the frequency of GG genotype in ApoH exon 8was74.5%,GC genotype was25.5%,the frequency of G allele was 87%,the frequency of GC genotype and C allele in CHD group were significant high than that in control group.LDL level in CHD group was significant high than that in control group(P0.05).LDL level of exon 3 in CHD group was significant high than that in control group(P0.05).But there were no significant difference among the level of lipid in the genotype of CHD and in the control group.LDL level of exon 8 in CHD group was significant high than that in control group(P(0.05).)TG level in genotype GC of CHD was significant high than that either in GG genotype of CHD or in any genotype of the control group.Conclusion The polymorphism of the apolipoprotein H exon 3 has no relationship with CHD,but the GC genotype of ApoH exon 3 and C allele have close relation with CHD and serum TG level.The polymorphism of the apolipoprotein H exon 3 has no significant relationship with the level of serum lipid.But there are close relation between polymorphism of the apolipoprotein H exon 8 and serum TG level.