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2009•Yunnan Journal of Traditional Chinese Medicine and Materia MedicaRequires access

Determination of Emodin and Chrysophanol in Jinyingdanshu Granules by HPLC

Zhu Zheng-yu

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Abstract

Objectives:To establish a method for the determination of emodin and chrysophanol in Jinyingdanshu Granules by HPLC. Method:The column was Shim Pack C18 and the mobile phase was melhanol 0.1% phosphate acid (80:20),with the detecting wavelength at 254 nm. Result:The recovery rate of Emodin was 99.63%,RSD=0.79% while the recovery rate of Chrysophanol was 98.10%,RSD=2.04%. Conclusion:The method is simple and quick with good reappaerance,and can be used for the quantitative analysis of Emodin and Chrysophanol.

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What this paper is about

Objectives:To establish a method for the determination of emodin and chrysophanol in Jinyingdanshu Granules by HPLC. Method:The column was Shim Pack C18 and the mobile phase was melhanol 0.1% phosphate acid (80:20),with the detecting wavelength at 254 nm. Result:The recovery rate of Emodin was 99.63%,RSD=0.79% while the recovery rate of Chrysophanol was 98.10%,RSD=2.04%. Conclusion:The method is simple and quick with good reappaerance,and can be used for the quantitative analysis of Emodin and Chrysophanol.

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Available abstract

Objectives:To establish a method for the determination of emodin and chrysophanol in Jinyingdanshu Granules by HPLC. Method:The column was Shim Pack C18 and the mobile phase was melhanol 0.1% phosphate acid (80:20),with the detecting wavelength at 254 nm. Result:The recovery rate of Emodin was 99.63%,RSD=0.79% while the recovery rate of Chrysophanol was 98.10%,RSD=2.04%. Conclusion:The method is simple and quick with good reappaerance,and can be used for the quantitative analysis of Emodin and Chrysophanol.

Key concepts: Emodin, Chromatography, High-performance liquid chromatography, Chemistry, Recovery rate

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