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Determination of Emodin and Chrysophanol in Yinhu Qinggan Granules by HPLC

Lisheng Wang

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Abstract

Objective:To establish a method for determination of the contents of emodin and chrysophanol in Yinhu Qinggan Granules.Methods:HPLC medthod was selected to determine the contents of emodin and chrysophanol.The chromatographic conditions included lnertsil ODS-3 C_(18)column,methanol-(0.1%)phosphoric acid solution(90∶10)as the mobile phase,wavelength of detector at 254 nm,flow rate 1.0 ml/min and column temperature at room temperature.Results:The calibration curves were linear over the range of 69.76～348.80 ng(r=0.999 6)for emodin and 12.08～60.40 ng(r=0.999 9) for chrysophanol;and the average recoveries were 98.97%(RSD=1.16%)and 99.72%(RSD=2.06%).Conclusion:This method is simple,accurate and reliable,which could be used for determination of the contents of emodin and chrysophanol in Yinhu Qinggan granules.

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What this paper is about

Objective:To establish a method for determination of the contents of emodin and chrysophanol in Yinhu Qinggan Granules.Methods:HPLC medthod was selected to determine the contents of emodin and chrysophanol.The chromatographic conditions included lnertsil ODS-3 C_(18)column,methanol-(0.1%)phosphoric acid solution(90∶10)as the mobile phase,wavelength of detector at 254 nm,flow rate 1.0 ml/min and column temperature at room temperature.Results:The calibration curves were linear over the range of 69.76～348.80 ng(r=0.999 6)for emodin and 12.08～60.40 ng(r=0.999 9) for chrysophanol;and the average recoveries were 98.97%(RSD=1.16%)and 99.72%(RSD=2.06%).Conclusion:This method is simple,accurate and reliable,which could be used for determination of the contents of emodin and chrysophanol in Yinhu Qinggan granules.

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Available abstract

Objective:To establish a method for determination of the contents of emodin and chrysophanol in Yinhu Qinggan Granules.Methods:HPLC medthod was selected to determine the contents of emodin and chrysophanol.The chromatographic conditions included lnertsil ODS-3 C_(18)column,methanol-(0.1%)phosphoric acid solution(90∶10)as the mobile phase,wavelength of detector at 254 nm,flow rate 1.0 ml/min and column temperature at room temperature.Results:The calibration curves were linear over the range of 69.76～348.80 ng(r=0.999 6)for emodin and 12.08～60.40 ng(r=0.999 9) for chrysophanol;and the average recoveries were 98.97%(RSD=1.16%)and 99.72%(RSD=2.06%).Conclusion:This method is simple,accurate and reliable,which could be used for determination of the contents of emodin and chrysophanol in Yinhu Qinggan granules.

Key concepts: Emodin, Chromatography, Phosphoric acid, Chemistry, High-performance liquid chromatography, Calibration curve, Linear range, Methanol

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