Effects of Exercise and Resveratrol on the Changes of Skeletal Muscle Mitochondria of Mouse

Objective:To observe the effect of resveratrol on exercise-induced skeletal muscle mitochondrial morphology and mitochondrial Ca2+ change.Methods:70 male Kunming mice were randomly divided into three groups:control group(CG),exercise group(EG)and exercise+resveratrol group(ER),and EG group and ER group Were divided into three sub-groups by post-exercise 0 hour,24 hours,48 hours,which every group has 10 mice.The mice of group ER were given resveratrol with 10 mg(/kg·d),and taken protocol of treadmill training for 4 weeks,then had a gradient of 0 and a speed of 18m/ min,continued 90 min of treadmill exercise as did the mice of group EG.Mice were slay respectively.The histological change of mitochondrial were observed by electron microscope.The activity of muscle SOD and MDA,concentration of muscle mitochondria Ca2+ were measured using Ultraviolet Spectrometer.Results:The electron microscope results showed that EG appeared swelling,cavitation,myeloid and some of the mitochondria were irregularly arranged,ER mice Skeletal muscle mitochondria with ridges,membrane fusion,fuzzy unclear after exercise,and the injury level of ER was less than EG.skeletal muscle of ER group SOD activity was significantly increased,MDA with significantly decrease compared with the EG group,a significant difference(P0.05).Compare with CG,the concentration of mitochondria Ca2+ was significantly increased in EG,especially post-exercise 24 h(P0.05).The concentration of mitochondria Ca2+ of ER was significantly lower than that of EG(P0.05).Conclusion:After the long time treadmill exercise,the muscle mitochondrial presents the obvious damage change,supplement resveratrol can improve mice antioxidant capacity,lower lipid peroxidation,and regulation of muscle mitochondrial Ca2+ concentration,so resveratrol could have a certain protective effect on structure and function of mitochondria.