Optimization of Ultrasound-assisted Extraction of Polysaccharides from Okra by Response Surface Analysis

Objective:To optimize the ultrasound-assisted extraction of polysaccharides from okra by response surface analysis. Methods:On the basis of single-factor experiments,the relationship between the yield of polysaccharides and main extraction conditions including extraction time,liquid/solid ratio and temperature was modeled using a 3-factor,3-level Box-Behnken experimental design and further,the established model was analyzed by response surface methodology to obtain the optimum extraction conditions.Results:The optimum extraction conditions were obtained as follows:extraction time of 20 min,liquid/ solid ratio of 44:1 and temperature of 52℃.Under these conditions,the estimated and observed values of maximal yield of polysaccharides were 27.82%and 27.75%,respectively.Conclusion:The results provide a reference for extraction of okra polysaccharides,and lay a foundation for future research on okra.