Optimization of Ultrasonic-assisted Extraction of Polysaccharides from Fructus Mori by Response Surface Methodology

In the present study,the conditions for ultrasonic-assisted extraction of polysaccharides from Fructus Mori were optimized by response surface analysis. On the basis of single-factor experiments,the relationship between main extraction conditions including extraction time,the ratio of water to raw material and extraction temperature were modeled using a 3-factor,3-level Box-Behnken experimental design. The established model was analyzed by response surface methodology to obtain the optimum extraction conditions. The results showed that the optimum extraction conditions were obtained as follows: extraction time was 23. 5 min,the ratio of water to raw material was 27∶ 1( v∶ m,mL / g) and extraction temperature was 72 ℃. Under these conditions,the estimated and observed values of maximal extraction yield of polysaccharides were 17. 80% and 17. 78 ± 0. 85%( n = 3),respectively. The method had the advantage of time-saving and efficient compared with the traditional water extraction. The results reported in this study provided the experimental basis for the follow-up research and development of Fructus Mori polysaccharides.