Optimization of Extraction Process of Polysaccharides from Fenugreek (Trigonella foenum-graecum L.) by Response Surface Methodology

Single-factor tests and response surface method(RSM) were used to optimize the extraction process of fenugreek seed polysaccharides(FSP).In order to obtaining the maximum of extraction yield of the polysaccharides,three main factors including extraction temperature,extraction times and ratio of raw material to water were selected with the Box-Behnken design and a quadric regression equation for predicting the yield of polysaccharides was established.The results showed that the optimum extraction conditions were as follows: extraction temperature 85 ℃,extraction times 1.2 h,and ratio of raw material to water 1∶ 28.Under the conditions,the optimum extracting yield of FSP was 19.89%,which is well matched with the predictive extracting yield 20.24%.