2003•Journal of Biological ControlRequires access

Inhibitive Substance Produced by Bacillus subtilis B2 Strain

Shiyi Yao

Abstract

Liquid culture of Bacillus subtilis B2 strain was precipitated by HCI, and extracted with methanol. Crude extract was separated by HPLC on Xterra RP18 column, and 120 fractions were collected. Bioassay of each fraction was carried out by detection of inhibition effect on conidial germination of Fusarium graminearum. LCMS analysis indicated that the inhibition substance were lipopeptide surfactin, polyene and an unreported compound.

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Key concepts: Bacillus subtilis, Surfactin, Lipopeptide, Strain (injury), Bioassay, Chromatography, Biology, High-performance liquid chromatography