Mechanism for reversing left ventricular remodeling of spontaneously hypertensive rats(SHRs) by valsartan and pancreatic kininogenase

Objective To investigate the combined effects of kallikrein and valsartan(Val) on reversal of left ventricular remodeling,angiotensin Ⅱ(Ang Ⅱ) and serum nitric oxide(NO) level in spontaneously hypertensive rats(SHRs).Methods Twenty-four 15 week-old SHRs and 8 fifteen-week male Wistar-Kyoto rats(WKYs) were divided randomly into SHR group,pancreatic kininogenase(PK) group,Val+PK group and WKY group to conduct experiment lasting for 4 weeks.Ang Ⅱlevels were detected by radioimmunology;plasma NO contents were determined by chemical method;systolic blood pressure(SBP),left ventricular mass index(LVMI), collagen volume fraction(CVF) and perivascular collagen area(PVCA) were detected by routine method.Results In SHR group,SBP,LVMI,CVF,PVCA and Ang Ⅱ were higher,serum NO level was decreased obviously than those of WKY group(P0.01).In each treatment group,serum NO level increased(P0.01) obviously,other indexes obviously reduced(P0.01) than those in SHR control group significantly(P0.01).Plasma Ang Ⅱ levels increased significantly(P0.01),myocardium Ang Ⅱ levels decreased(P0.01) significantly in Val+PK group.But there was no obvious change in Ang Ⅱ levels in PK group.Conclusions Val and PK all could effectively ameliorate left ventricular remodeling of hypertension,especially combined Val and PK.