Effect of pancreatic kininogenase on expression of TGF-β1 and nitrol oxide level in spontaneously hypertensive rats

Objective To investigate the effect of pancreatic kininogenase on the expression of TGF-β1 and nitrol oxide(NO) level in myocardium of spontaneously hypertensive rats(SHRs) and to explore its pharmacological mechanism for reducing blood pressure and reversing left ventricular remodeling.Methods 24 male fifteen-week SHRs were randomly divided into SHR group,pancreatic kininogenase group,captoprill group(n=8 in each),8 male fifteen-week Wistar Kyoto(WKY) was selected as control group.After four weeks,blood pressure of all the rats were measured through carotid artery intubation to detect serum NO level.Then the rats were sacrificed to determine left ventricular mass index(LVMI) and stain myocardial tissue with VE and to detect collagen volume fraction(CVF).TGF-β1 expressions were detected by immunohistochemical technique(SP method).Results In SHRs group,systolic blood pressure(SBP),LVMI,CVF,perivascular circumferential area(PVCA) and expression of TGF-β1 were higher,serum NO level was decreased obviously than those in WKY group(P0.05).After using pancreatic kininogenase therapy,serum NO level was raised,and closed to normal level,all other indexes were decreased obviously than those in SHR group(P0.05).There was no difference between pancreatic kininogenase group and captoprill group except SBP.Conclusions Pancreatic kininogenase could obviously increase NO level and suppress the expression of TGF-β1 in myocardial tissue,by which reduces ventricular remodeling in SHRs.