Effect of Pancreatic Kininogenase on Ventriculer Remodeling in Spontaneously Hypertensive Rats

Objective To investigate the effect of pancreatic kininogenase on the expression of matrix metalloproteinases-2 2(MMP-2), transfer growth factor-β_ 1 (TGF-β_ 1 ) and ventriculer remodeling in spontaneously hypertensive rats (SHR). Methods Twenty-four male 15 weeks SHR were randomly divided into three groups: SHR group, pancreatic kininogenase treatment group(PK: 7.2 U/kg·d), captopril treatment group(Cap: 10 mg/kg·d)(n=8 in each), 8 Wister Kyoto were served as control. After four weeks, blood pressure were measured througth carotid artery catherization. Myocardial tissue was stained with VG and pathological changes were studied. MMP-2, TGF-β_ 1 were determined by immunohisto-chemical technique(SP method). Results In pancreatic kininogenase treated SHR, SBP(183±12 vs SHR: 234±23)mm Hg, LVMI(2.89±0.15 vs SHR: 3.06±0.18)mg/g, CVF(0.17±0.03 vs SHR: 0.26±0.05)%, PVCA(0.57±0.26 vs SHR: 0.99±0.47)% and expression of MMP-2, TGF-β_ 1 in SHR were significantly improved (P0.05). All indexs were significantly decresed, and were close to captopril group. Conclusion Pancreatic kinigenase significantly reversed LVMI, CVF and PVCA, which may be mediated by attenuating expression of MMP-2 and TGF-β_1 in myocardial tissue.