Detection of interleukin-3 and interleukin-8 levels in plasma of aplastic anemia patients

Objective:To observe changes of interleukin-3(IL-3) and interleukin-8(IL-8) levels and discuss its significance in pathogenesis of aplastic anemia(AA).Methods:The method of ELISA was used to detect IL-3 and IL-8 levels in plasma of 30 AA patients and 10 normal volunteers.Results:There was no significant difference of plasma IL-3 levels between the AA patients and controls(P0.05);Compared with groups of the normal controls and the patients with PIG-A gene mutation,the levels of plasma IL-8 significantly increased in the group of AA patients without PIG-A gene mutation(P0.01);There was no significant difference of plasma IL-8 levels between the group of AA patients with PIG-A mutation and the normal controls(P0.05).Conclusions:There are cellular immune dysfunction in patients with AA.Abnormal levels of IL-8 might be involved in the pathogenesis of AA and high level of IL-8 may closely linked to the occurrence of AA.