Inhibitory effect of lactuside B on TRPM7 mRNA expression in hippocampus and striatum in cerebral ischemic rats

OBJECTIVE To explore the effect of lactuside B on transient receptor potential melastatin 7(TRPM7) mRNA expression in rat hippocampus and striatum after cerebral ischemia injury.METHODS The middle cerebral artery occulsion was used to make the ischemia(2 h) reperfusion injury model.After reperfusion,the rats were respectively ip given lactuside B 12.5,25 and 50 mg·kg-1 twice daily.Half of the rats of each group administered for 1 d while the rest administered continuously for 3 d.After the neurologic deficit score was observed in the last administration,the rats were sacrificed.The expression of TRPM7 mRNA on hippocampus and striatum was detected by RT-PCR.RESULTS Compared with sham group,the neurological lack symptom score in model group was significantly higher and the TRPM7 mRNA expression increased in hippocampus and striatum in rats.After lactuside B 12.5,25 and 50 mg·kg-1 was administered for 1 d,TRPM7 mRNA expression was decreased in hippocampus and striatum(hippocampus: 0.68±0.02,0.55±0.02 and 0.56±0.02;striatum: 0.32±0.02,0.25±0.01 and 0.35±0.01).TRPM7 mRNA expression was lower after lactuside B administered for 3 d(hippocampus: 0.29±0.02,0.18±0.01 and 0.26±0.01;striatum: 0.28±0.02,0.19±0.01 and 0.27±0.01).CONCLUSION Lactuside B can reduce TRPM7 gene expression in hippocampus and striatum,suggesting that lactuside B play a positive therapeutic and protective role.