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2006•Di-san junyi daxue xuebaoRequires access

Expression of growth-associated protein in hippocampus of neonatal rats with hypoxic-ischemic brain damage

PU Zhao-xia

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Abstract

Objective To observe the expression of growth-associated protein-43(GAP-43) in the hippocampus of neonatal rats with hypoxic-ischemic brain damage (HIBD). Methods HIBD was established by the method of Rice in 48 SD rats aged 7 days and another 48 matched normal rats served as control. Immunohistochemistry and RT-PCR were respectively used to detect the expression of GAP-43 protein and mRNA in all rat hippocampus on day 8, 10, 14, 21, 28, 35 after HIBD (n=8 at each time point for each group). Results The expression of GAP-43 protein and mRNA in hippocampus was obviously increased in HIBD rats as compared with normal rats, the peak of GAP-43 protein appeared at 3rd week, and the peak of mRNA at 2nd week. Conclusion The expression of GAP-43 protein and mRNA increased in the hippocampus of rats following HIBD, which was possibly related to the recovery of injured hippocampus.

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What this paper is about

Objective To observe the expression of growth-associated protein-43(GAP-43) in the hippocampus of neonatal rats with hypoxic-ischemic brain damage (HIBD). Methods HIBD was established by the method of Rice in 48 SD rats aged 7 days and another 48 matched normal rats served as control. Immunohistochemistry and RT-PCR were respectively used to detect the expression of GAP-43 protein and mRNA in all rat hippocampus on day 8, 10, 14, 21, 28, 35 after HIBD (n=8 at each time point for each group). Results The expression of GAP-43 protein and mRNA in hippocampus was obviously increased in HIBD rats as compared with normal rats, the peak of GAP-43 protein appeared at 3rd week, and the peak of mRNA at 2nd week. Conclusion The expression of GAP-43 protein and mRNA increased in the hippocampus of rats following HIBD, which was possibly related to the recovery of injured hippocampus.

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Available abstract

Objective To observe the expression of growth-associated protein-43(GAP-43) in the hippocampus of neonatal rats with hypoxic-ischemic brain damage (HIBD). Methods HIBD was established by the method of Rice in 48 SD rats aged 7 days and another 48 matched normal rats served as control. Immunohistochemistry and RT-PCR were respectively used to detect the expression of GAP-43 protein and mRNA in all rat hippocampus on day 8, 10, 14, 21, 28, 35 after HIBD (n=8 at each time point for each group). Results The expression of GAP-43 protein and mRNA in hippocampus was obviously increased in HIBD rats as compared with normal rats, the peak of GAP-43 protein appeared at 3rd week, and the peak of mRNA at 2nd week. Conclusion The expression of GAP-43 protein and mRNA increased in the hippocampus of rats following HIBD, which was possibly related to the recovery of injured hippocampus.

Key concepts: Hippocampus, Gap-43 protein, Brain damage, Messenger RNA, Internal medicine, Endocrinology, Immunohistochemistry, Protein expression

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