Effect of Baicalin on Oxygen-Glucose Deprivation/Reperfusion-Induced Injury in Mouse Brain Slices and Cultured Rat Cortical Neurons

OBJECTIVE To investigate the protective effect of baicalin on oxygen-glucose deprivation/reperfusion(OGD/RP)-induced injury and possible mechanism in mouse brain slices and rat cortical neurons.METHODS In mouse brain slices,the viability after OGD/RP was assessed by the formation of formazn,a red product of 2,3,5-trihenylterzolium chloride (TTC).In primary cultured rat cortical neurons,the neuronal viability and damage after OGD/RP were assessed by MTT reduction and LDH release,and the intracellular free radicals were measured with 2,7-dichlorofluorescein diacetate(DCF-DA).The effect of baicalin at different times on OGD/RP injury at various concentrations was evaluated.RESULTS In mouse brain slices,baicalin significantly attenuated OGD/RP injury at 0.01～1 μmol·L~(-1) when baicalin was added throughout the experiment or during OGD,and at 0.1～1 μmol·L~(-1) when baicalin was added during reperfusion.In rat cortical neurons,baicalin protected the neurons from OGD/RP injury,and inhibited the elevation of intracellular free radicals at 0.01～1 μmol·L~(-1) when baicalin was added throughout the experiment.CONCLUSION Baicalin had concentration-dependently protective effect on OGD/RP injury in vitro,even when being administrated during reperfusion,this effect may be,at least partly,mediated by the inhibition of intracellular free radicals.