Protective effects of resveratrol on injury induced by oxygen-glucose deprivation in the primary cultured cortical neurons of neonatal rats

OBJECTIVE To investigate the protective effects of resveratrol(Res) on injury induced by oxygen-glucose deprivation(OGD) in the primary cultured neurons of neonatal rats.METHODS In the 10th day since cerebral cortical neurons of neonatal rats were cultured,OGD model was induced by low carbohydrates plus physical hypoxia,and reperfused with oxygen-glucose deprivation(RP) 2 h later.In the experiment,Nimodipine(Nim,1.0 μmol·L-1) was used as positive control,MTT reduction and the release rate of lactate dehydrogenase(LDH) were measured at the different time of OGD 1,2,4 h,OGD 2 h/RP 3,12,24 h.Morphologic change of neurons were observed by light micsoscope and electron microscope.And the effects of Res(0.1,1.0,10.0 μmol·L-1) on injury induced by OGD and OGD/RP were determined.RESULTS MTT reduction and the release of LDH were ameliorated by pretreatments with Res at OGD 1,2,4 h,OGD 2 h/RP 3,12,24 h,and pretreatments with Res ameliorated the morphologic changes of neurons induced by OGD and OGD/RP.CONCLUSION Res may protect against injury induced by OGD in the primary cultured neurons of neonatal rats