Protective Effect of Baicalin on Slice Injury Induced by Oxygen Glucose Deprivation

Objective To investigate the protective effect and its mechanisms of Baicalin on slice injury induced by oxygen glucose deprivation(OGD) on rat brain slices.Methods The rat slice injury models were established by OGD or glutamate(Glu) stress and randomly divided into groups as follows: control group,OGD or glutamate injury group and Baicalin 10 mg/L and 30 mg/L group.The histological changes and absorbent value of brain slices determined by HE staining and TTC staining,and lactate dehydrogenase(LDH) release in incubation medium were used to evaluate the protective effect of different concentrations of Baicalin on brain injury.Results Compared with injury groups,10 mg/L and 30 mg/L Baicalin inhibited the decrease of absorbent value at 490 nm of slices and the increase of LDH release induced by OGD or Glu,ameliorated the pathological injury of cortical neuron induced by OGD.Conclusion Baicalin possesses protective effect on OGD injury in rat brain slices,which is related to its inhibitory effect on excitatory amino acids toxicity.