Effect of EVn-50 on Proliferation and Apoptosis of the Human Cervical Carcinoma Cell line Hela

Objective To investigate the effect of EVn50 on the proliferation and apoptosis of human cervical carcinoma(Hela) cell line in vitro.Methods Hela cells were cultured in vitro.The trypan blue exclusion method was used to determine the effect of EVn-50 on the proliferation of Hela cells.Plate-colon and Soft ager-colon assay was used to test colon formation inhibitory effect of Hela by EVn-50.AO/EB fluorescence staining was used to observed the morphologic changes of apoptosis induced by EVn-50 in Hela cell line.DNA agarose gel electrophoresis was used to test apoptosis induced by EVn-50 in Hela cell line.Results EVn-50 significantly inhibited human cervical carcinoma Hela cell lines proliferation in vitro in a dose-dependent manner.Typical morphologic changes of apoptosis could be observed after treatment with EVn-50 by fluorescence microscope using AO/EB fluorescence staining.DNA agarose gel electrophoresis shown that DNA ladder bands could appear after treatment with EVn-50 at 100 μg/mL for 48 h.Conclusion EVn-50 can significantly inhibit Hela cells proliferation and induce apoptosis.