Effect of 8-nitrorchrysin on inhibition of growth and induction of apoptosis in human uterine cervix cancer Hela cells in vitro

Objective To investigate the effect of 8-nitrochrysin(8-NOChR) on the inhibition of the growth and induction of apoptosis in human uterine cervix cancer Hela cells cultured in vitro.Methods Hela cells was cultured in vitro.MTT assay was used to determine the proliferation of Hela cells,soft agar clone-formation method was used to determine the growth of Hela cells,AO/EB fluorescence staining was used to observe the morphologic changes of apoptosis in Hela cell line.DNA agarose gel electrophoresis was used to test the apoptosis of Hela cell line.Results MTT assay showed 8-NOChR significantly suppressed proliferation of Hela cell line in a dose-dependent manner.Soft agar clone-formation assay showed 8-NOChR obviously inhibited clone-formation of Hela cells.Typical morphologic changes of apoptosis were observed after the treatment with 8-NOChR by fluorescence microscope using AO/EB staining.DNA ladder bands appeared in DNA agarose gel electrophoresis after the treatment with 8-NOChR at 30μmol/L for 72h.Conclusion 8-NOChR could inhibit the proliferation and induce the apoptosis of human uterine cervix cancer Hela cells.