Study on preparation of ginsenoside-Rd solid lipid nanoparticles

OBJECTIVE To develop ginsenoside-Rd solid lipid nanoparticles(Rd-SLN) encapsulation.METHODS To optimize the technique of preparation,Rd-SLN was prepared by film-ultrasonic wave dissolving technique,emulsion evaporation and solidification,high shear homogenization and ultrasound,and high pressure homogenization,respectively.Based on the test of single factor,the optimized formulation of Rd-SLN was conveniently obtained by the L9(34) orthogonal methodology.The entrapment efficiency was determined by sephadex gel chromatography and high performance liquid chromatography.The electron microscope was used to observe the surface of Rd-SLN by negative-dyeing.Particle characterization system and zeta potential analyzer were used to study the particle diameter and Zeta potential of Rd-SLN in suspension.RESULTS The Rd-SLN was prepared suitably by high pressure homogenization.The particulate of the SLN was regular ellipse,symmetrical.The mean particle size was (102.7±27.0)nm(n=3),and the Zeta potential was(-44.9±9.5)mV(n=3).The entrapment efficiency and drug loading capacity of the SLN sample was(81.8±2.6) % and(6.37±0.21) %(n=3) respectively.CONCLUSION The technique of preparing ginsenoside-Rd solid lipid nanoparticles by high pressure homogenization is stable and feasible.